plenti cmv puro dest empty vector (Addgene inc)
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plenti cmv puro dest empty vector
Plenti Cmv Puro Dest Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/pLenti+CMV+Puro+DEST+(w118-1)+(Plasmid+%2317452)/bio_rxiv__64898__2026__03__28__714855-261-8-23
Average 96 stars, based on 330 article reviews
Plenti Cmv Puro Dest Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/pLenti+CMV+Puro+DEST+(w118-1)+(Plasmid+%2317452)/bio_rxiv__64898__2026__03__28__714855-261-8-23
Average 96 stars, based on 330 article reviews
plenti cmv puro dest empty vector - by Bioz Stars,
2026-09
96/100 stars
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Transfection:Article Title: K-Homology Splicing Regulatory Protein (KSRP) Augments Survival and Proliferation of Human Melanoma Cells Article Snippet: Cells were seeded in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in 5% CO 2 . .. For KSRP knoc down, 293 T cells (5 × 10 6 /well) were transfected with KSRP shRNA (Dharmacon A Horizon Discovery Group company, Lafayette, CO, USA) or Article Title: Spatial tumor immune heterogeneity facilitates subtype co-existence and therapy response in pancreatic cancer Article Snippet: .. Patient-derived primary cell line GCDX62 was maintained in a 3:1 mixture of Keratinocyte-SFM (KSF; Thermo Fisher Scientific; supplemented with 2% (v/v) FCS, 1% (v/v) Penicillin-streptomycin, bovine pituitary extract (BPE), and human epidermal growth factor) and RPMI 1640 containing 10% (v/v) FCS. cJUN overexpression (cJUN-OE; construct pMSCV-cJUN, no. 34898, addgene) and Article Title: Isoform specific regulation of osteopontin by AKT2 in hepatocytes and livers. Article Snippet: Elevated levels of osteopontin (OPN), an inflammatory cytokine, are correlated with chronic inflammatory conditions and liver cancer.. In this study, we explored the regulation of OPN in liver and hepatocytes by AKT1 vs. AKT2, the two AKT isoforms expressed in hepatocytes and livers.. Using a mouse model lacking PTEN (phosphatase and tensin homologue deleted on chromosome 10), the negative regulator of phosphatidylinositol 3-kinase (PI3K)/AKT signaling, expression of secreted phosphoprotein 1 (Spp1), the gene encoding OPN, was found to be the topmost significantly upregulated gene in the liver. shRNA:Article Title: K-Homology Splicing Regulatory Protein (KSRP) Augments Survival and Proliferation of Human Melanoma Cells Article Snippet: Cells were seeded in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in 5% CO 2 . .. For KSRP knoc down, 293 T cells (5 × 10 6 /well) were transfected with KSRP shRNA (Dharmacon A Horizon Discovery Group company, Lafayette, CO, USA) or Over Expression:Article Title: Spatial tumor immune heterogeneity facilitates subtype co-existence and therapy response in pancreatic cancer Article Snippet: .. Patient-derived primary cell line GCDX62 was maintained in a 3:1 mixture of Keratinocyte-SFM (KSF; Thermo Fisher Scientific; supplemented with 2% (v/v) FCS, 1% (v/v) Penicillin-streptomycin, bovine pituitary extract (BPE), and human epidermal growth factor) and RPMI 1640 containing 10% (v/v) FCS. cJUN overexpression (cJUN-OE; construct pMSCV-cJUN, no. 34898, addgene) and Construct:Article Title: Spatial tumor immune heterogeneity facilitates subtype co-existence and therapy response in pancreatic cancer Article Snippet: .. Patient-derived primary cell line GCDX62 was maintained in a 3:1 mixture of Keratinocyte-SFM (KSF; Thermo Fisher Scientific; supplemented with 2% (v/v) FCS, 1% (v/v) Penicillin-streptomycin, bovine pituitary extract (BPE), and human epidermal growth factor) and RPMI 1640 containing 10% (v/v) FCS. cJUN overexpression (cJUN-OE; construct pMSCV-cJUN, no. 34898, addgene) and Plasmid Preparation:Article Title: Spatial tumor immune heterogeneity facilitates subtype co-existence and therapy response in pancreatic cancer Article Snippet: .. Patient-derived primary cell line GCDX62 was maintained in a 3:1 mixture of Keratinocyte-SFM (KSF; Thermo Fisher Scientific; supplemented with 2% (v/v) FCS, 1% (v/v) Penicillin-streptomycin, bovine pituitary extract (BPE), and human epidermal growth factor) and RPMI 1640 containing 10% (v/v) FCS. cJUN overexpression (cJUN-OE; construct pMSCV-cJUN, no. 34898, addgene) and Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner. Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of SpyCas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sgSSEA, Additional file 1: Table S4) or Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of Spy Cas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sg SSEA , Additional file 1: Table S4) or Article Title: The TET3/GATA6 Axis Drives Lipid Metabolism and Therapeutic Vulnerabilities in Pancreatic Ductal Adenocarcinoma Article Snippet: .. Article Title: Isoform specific regulation of osteopontin by AKT2 in hepatocytes and livers. Article Snippet: Elevated levels of osteopontin (OPN), an inflammatory cytokine, are correlated with chronic inflammatory conditions and liver cancer.. In this study, we explored the regulation of OPN in liver and hepatocytes by AKT1 vs. AKT2, the two AKT isoforms expressed in hepatocytes and livers.. Using a mouse model lacking PTEN (phosphatase and tensin homologue deleted on chromosome 10), the negative regulator of phosphatidylinositol 3-kinase (PI3K)/AKT signaling, expression of secreted phosphoprotein 1 (Spp1), the gene encoding OPN, was found to be the topmost significantly upregulated gene in the liver. Article Title: The TET3/GATA6 Axis Drives Lipid Metabolism and Therapeutic Vulnerabilities in Pancreatic Ductal Adenocarcinoma Article Snippet: GATA6 expression vector (Addgene, #120445) was packaged into a lentivirus transduction system and used to infect cancer cells. .. Control:Article Title: Spatial tumor immune heterogeneity facilitates subtype co-existence and therapy response in pancreatic cancer Article Snippet: .. Patient-derived primary cell line GCDX62 was maintained in a 3:1 mixture of Keratinocyte-SFM (KSF; Thermo Fisher Scientific; supplemented with 2% (v/v) FCS, 1% (v/v) Penicillin-streptomycin, bovine pituitary extract (BPE), and human epidermal growth factor) and RPMI 1640 containing 10% (v/v) FCS. cJUN overexpression (cJUN-OE; construct pMSCV-cJUN, no. 34898, addgene) and Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of Spy Cas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sg SSEA , Additional file 1: Table S4) or Article Title: The TET3/GATA6 Axis Drives Lipid Metabolism and Therapeutic Vulnerabilities in Pancreatic Ductal Adenocarcinoma Article Snippet: .. Article Title: The TET3/GATA6 Axis Drives Lipid Metabolism and Therapeutic Vulnerabilities in Pancreatic Ductal Adenocarcinoma Article Snippet: GATA6 expression vector (Addgene, #120445) was packaged into a lentivirus transduction system and used to infect cancer cells. .. Generated:Article Title: Spatial tumor immune heterogeneity facilitates subtype co-existence and therapy response in pancreatic cancer Article Snippet: .. Patient-derived primary cell line GCDX62 was maintained in a 3:1 mixture of Keratinocyte-SFM (KSF; Thermo Fisher Scientific; supplemented with 2% (v/v) FCS, 1% (v/v) Penicillin-streptomycin, bovine pituitary extract (BPE), and human epidermal growth factor) and RPMI 1640 containing 10% (v/v) FCS. cJUN overexpression (cJUN-OE; construct pMSCV-cJUN, no. 34898, addgene) and Incubation:Article Title: Spatial tumor immune heterogeneity facilitates subtype co-existence and therapy response in pancreatic cancer Article Snippet: .. Patient-derived primary cell line GCDX62 was maintained in a 3:1 mixture of Keratinocyte-SFM (KSF; Thermo Fisher Scientific; supplemented with 2% (v/v) FCS, 1% (v/v) Penicillin-streptomycin, bovine pituitary extract (BPE), and human epidermal growth factor) and RPMI 1640 containing 10% (v/v) FCS. cJUN overexpression (cJUN-OE; construct pMSCV-cJUN, no. 34898, addgene) and Clonogenic Cell Survival Assay:Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner. Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of SpyCas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sgSSEA, Additional file 1: Table S4) or Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of Spy Cas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sg SSEA , Additional file 1: Table S4) or Activity Assay:Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner. Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of SpyCas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sgSSEA, Additional file 1: Table S4) or Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of Spy Cas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sg SSEA , Additional file 1: Table S4) or Bacteria:Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner. Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of SpyCas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sgSSEA, Additional file 1: Table S4) or Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of Spy Cas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sg SSEA , Additional file 1: Table S4) or Transformation Assay:Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner. Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of SpyCas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sgSSEA, Additional file 1: Table S4) or Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of Spy Cas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sg SSEA , Additional file 1: Table S4) or Electroporation:Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner. Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of SpyCas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sgSSEA, Additional file 1: Table S4) or Article Title: Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner Article Snippet: .. A The schematic diagram of two-plasmid-based bacterial survival assay for detecting the activity of Spy Cas9 in bacteria. pCas plasmid (Addgene #62225,Additional file 1: Table S2) was transformed into the E. coli MG1655 before an electroporation with pTarget plasmids coding for sgRNA targeting SSEA (pT-sg SSEA , Additional file 1: Table S4) or Knockdown:Article Title: Interdependence of Pasha and Drosha for localization and function of the Microprocessor in C. elegans. Article Snippet: A Discovery V8 Stereo Microscope (Zeiss) was used for selection of mutagenized animals. .. For RNAi knockdown, synchronized L1 larvae were placed on RNAi plates containing IPTG and E. coli HT115 expressing dsRNA matching cdk-9 (Ahringer or Vidal libraries, H25P06.2), ama-1 (Ahringer library, F36A4.7), alg-1/alg-2 (Ahringer library, F48F7.1), pash-1 (Ahringer Nature Communications | (2025) 16:5595 13 library, T22A3.5), drsh-1 (Ahringer library, F26E4.10), or Expressing:Article Title: Interdependence of Pasha and Drosha for localization and function of the Microprocessor in C. elegans. Article Snippet: A Discovery V8 Stereo Microscope (Zeiss) was used for selection of mutagenized animals. .. For RNAi knockdown, synchronized L1 larvae were placed on RNAi plates containing IPTG and E. coli HT115 expressing dsRNA matching cdk-9 (Ahringer or Vidal libraries, H25P06.2), ama-1 (Ahringer library, F36A4.7), alg-1/alg-2 (Ahringer library, F48F7.1), pash-1 (Ahringer Nature Communications | (2025) 16:5595 13 library, T22A3.5), drsh-1 (Ahringer library, F26E4.10), or |
